Thursday, February 13, 2025

 Basic Microbiology Instruments:

·       Microscope – Used to magnify and observe microorganisms that are not visible to the naked eye.

·       Autoclave – Sterilizes media, glassware, and instruments using high-pressure steam.

·       Incubator – Provides optimal temperature and conditions for microbial growth.

·       Laminar Air Flow (LAF) Cabinet – Maintains a sterile environment for culturing microorganisms.

·       Hot Air Oven – Used for dry heat sterilization of glassware and metal instruments.

·       Centrifuge – Separates components of a mixture based on density by spinning at high speeds.

·       Spectrophotometer – Measures the absorbance of light to determine microbial growth.

·       pH Meter – Measures the acidity or alkalinity of a solution.

·       Colony Counter – Counts bacterial colonies grown on agar plates.

·       Bunsen Burner – Provides an open flame for sterilization and aseptic techniques.

·       Inoculating Loop – Transfers microorganisms in culture work.

·       Petri Dishes – Holds solid media (agar) for culturing microorganisms.

·       Glass Slides and Cover Slips – Used for microscopic examination of microorganisms.

·       Haemocytometer – Counts microbial cells under a microscope.

·       Anaerobic Jar – Creates an oxygen-free environment for anaerobic bacterial growth.

·       Refrigerator – Stores microbial cultures and temperature-sensitive reagents.

·       Vortex Mixer – Mixes small liquid samples quickly and efficiently.

·       Gas Pack System – Provides an anaerobic environment for the growth of anaerobes.

·       Shaking Incubator – Maintains cultures in motion for better oxygen distribution.

·       Staining Rack – Holds slides during staining procedures like Gram staining.

Glassware & Containers

·       Erlenmeyer Flask – Holds and mixes liquid culture media.

·       Beaker – Used for measuring and mixing liquids.

·       Test Tubes – Hold small volumes of liquid cultures for microbial growth.

·       Volumetric Flask – Measures precise liquid volumes for preparing solutions.

·       Pipettes (Micropipette & Glass Pipette) – Transfers precise liquid volumes.

·       Burette – Used for titrations to measure precise volumes.

Sterilization & Aseptic Equipment

·       Membrane Filter Unit – Filters and sterilizes solutions without heat.

·       UV Sterilizer – Uses ultraviolet light to sterilize work areas.

·       Sterile Forceps – Handles sterile materials without contamination.

·       Loop Sterilizer – Heats inoculating loops for sterilization.

Microbial Growth & Storage

·       Nutrient Agar Plates – Supports microbial growth in a solid medium.

·       Blood Agar Plates – Used for isolating and identifying hemolytic bacteria.

·       Slant Culture Tubes – Stores bacterial cultures for long-term use.

·       Deep Freezer (-80°C) – Stores bacterial and fungal strains for long-term preservation.

·       Cryovials – Stores bacterial cultures in liquid nitrogen or deep freeze conditions.

Identification & Biochemical Testing

·       API Test Strips – Identifies bacteria based on biochemical reactions.

·       ELISA Reader – Detects antigen-antibody reactions for microbial diagnostics.

·       PCR Machine (Thermal Cycler) – Amplifies DNA for microbial identification.

·       Gel Electrophoresis Unit – Separates DNA, RNA, or proteins for analysis.

·       Gas Chromatography (GC) – Identifies bacterial metabolites and fatty acids.

Environmental & Industrial Microbiology

·       Air Sampler – Captures airborne microorganisms for environmental monitoring.

·       Water Bath – Maintains constant temperatures for microbial incubations.

·       BOD Incubator – Maintains specific conditions for microbial analysis in wastewater testing.

·       Orbital Shaker – Gently mix cultures in liquid media.

·       Refractometer – Measures sugar concentrations in fermentation studies.

Miscellaneous Instruments

·       Hemolysis Loop – Identifies hemolysis patterns on blood agar plates.

·       Microtome – Slices thin sections of microbial biofilms for microscopy.

·       Fume Hood – Protects against harmful fumes during chemical preparations.

·       Magnetic Stirrer – Mixes solutions using a magnetic bar and rotating field.

·       Manometer – Measures gas pressure in fermentation and microbial respiration studies.

 

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